Mechanisms and Potential Therapeutic Use of Dihydromyricetin in Reducing Alcoholic Liver Disorder and Alcohol Abuse Disorder Behavior文献综述

Mechanisms and Potential Therapeutic Use of Dihydromyricetin in Reducing Alcoholic Liver Disorder and Alcohol Abuse Disorder Behavior

Mentor: Dr. Daryl L. Davies

1. Research Significance and Research Goal:

Dihydromyricetin (DHM), a bioactive flavanonol isolated from the Ampelopsis grossedentata species, is becoming recognized for its broad range of pharmacological properties, including antioxidative, anti-inflammatory, anticancer, antimicrobial, cell death-mediating and metabolism-mediating activities. Acting as an antioxidant, DHM can potentially counteract ethanol intoxication, reduce consumption, and protect cells against oxidative stress. To promote the efficacy and safety of DHM as a new therapeutic for the reduction/prevention of alcoholic liver disorder(ALD) and alcohol abuse disorder (AUD), we must first utilize in vitro studies to preliminarily elucidate its mechanisms and potential. By investigating DHM in these models, we will provide insight into the pharmacological benefits of DHM for a disorder with little to no effective treatment options.

1. Experimental Design
2. Cell Culture

Hep G2 cells and VL17A cells are cultured at 37℃ in a humidified atmosphere with 5% CO₂

1. MTT Cell Viability and Proliferation Assay

Test the viability and proliferation of Hep G2 cells and VL17A cells with or without DHM treatment in the presence of alcohol.

1. Fluorescent Staining and Imaging

Observe the amount of acidic vacuoles(autophagolysomes, lysosomes, and other lysome-related vacuoles) with or without DHM treatment in the presence of alcohol.

1. Western Blot Analysis

Analyze the expression of LCI, LC-II, Ulk1, p-Ulk1, mTOR, p-mTOR, PI3K85, PI3K110, Akt, p-Akt, Erk, p-Erk, AMPK, p-AMPK in Hep G2 cells and VL17A cells with or without DHM treatment in the presence of alcohol.

1. Blocking of Autophagy Pathway

Observe the effect of DHM in autophagy-blocked HepG2 and VL 17A cells.

1. Timeline

February: Read literature and design experiments